Single-step purification of dihydrofolate reductases on pteridine matrices
نویسندگان
چکیده
منابع مشابه
Dihydrofolate Reductase of Streptococcus f aecium II. PURIFICATION AND SOME PROPERTIES OF TWO DIHYDROFOLATE REDUCTASES FROM THE AMETHOPTERIN-RESISTANT
From a single amethopterin-resistant organism, Streptococcus faecium var. durans strain A, two different dihydrofolate reductases have been obtained as essentially homogeneous proteins in good yield. One of the reductases has a similar substrate specificity and turnover number (about 8000 moles per min per mole of enzyme) to the single reductase found in the amethopterin-sensitive strain of S. ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1972
ISSN: 0306-3283
DOI: 10.1042/bj1270028p